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\n \n\n \n \n Kamiya, C., Ohta, N., Ogura, Y., Yoshida, K., Horie, T., Kusakabe, T. G., Satake, H., & Sasakura, Y.\n\n\n \n \n \n \n \n Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: FUNCTIONS OF GNRH IN ASCIDIANS.\n \n \n \n \n\n\n \n\n\n\n Developmental Dynamics, 243(12): 1524–1535. December 2014.\n \n\n\n\n
\n\n\n\n \n \n \"NonreproductivePaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{kamiya_nonreproductive_2014,\n\ttitle = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: {FUNCTIONS} {OF} {GNRH} {IN} {ASCIDIANS}},\n\tvolume = {243},\n\tissn = {10588388},\n\tshorttitle = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176},\n\tdoi = {10.1002/dvdy.24176},\n\tlanguage = {en},\n\tnumber = {12},\n\turldate = {2021-07-27},\n\tjournal = {Developmental Dynamics},\n\tauthor = {Kamiya, Chisato and Ohta, Naoyuki and Ogura, Yosuke and Yoshida, Keita and Horie, Takeo and Kusakabe, Takehiro G. and Satake, Honoo and Sasakura, Yasunori},\n\tmonth = dec,\n\tyear = {2014},\n\tpages = {1524--1535},\n}\n
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\n \n\n \n \n Sasaki, H., Yoshida, K., Hozumi, A., & Sasakura, Y.\n\n\n \n \n \n \n \n CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis.\n \n \n \n \n\n\n \n\n\n\n Development, Growth & Differentiation, 56(7): 499–510. September 2014.\n \n\n\n\n
\n\n\n\n \n \n \"CRISPR/Cas9-mediatedPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{sasaki_crisprcas9-mediated_2014,\n\ttitle = {{CRISPR}/{Cas9}-mediated gene knockout in the ascidian \\textit{{Ciona} intestinalis}},\n\tvolume = {56},\n\tissn = {0012-1592, 1440-169X},\n\tshorttitle = {{\\textless}span style="font-variant},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149},\n\tdoi = {10.1111/dgd.12149},\n\tlanguage = {en},\n\tnumber = {7},\n\turldate = {2021-07-27},\n\tjournal = {Development, Growth \\& Differentiation},\n\tauthor = {Sasaki, Haruka and Yoshida, Keita and Hozumi, Akiko and Sasakura, Yasunori},\n\tmonth = sep,\n\tyear = {2014},\n\tpages = {499--510},\n}\n\n
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\n \n\n \n \n Sasakura, Y.\n\n\n \n \n \n \n \n Germline Transformation in the Ascidian Ciona intestinalis.\n \n \n \n \n\n\n \n\n\n\n In Sawada, H., Inoue, N., & Iwano, M., editor(s), Sexual Reproduction in Animals and Plants, pages 465–473. Springer Japan, Tokyo, 2014.\n \n\n\n\n
\n\n\n\n \n \n \"GermlinePaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@incollection{sawada_germline_2014,\n\taddress = {Tokyo},\n\ttitle = {Germline {Transformation} in the {Ascidian} \\textit{{Ciona} intestinalis}},\n\tisbn = {978-4-431-54588-0 978-4-431-54589-7},\n\turl = {http://link.springer.com/10.1007/978-4-431-54589-7_38},\n\tlanguage = {en},\n\turldate = {2021-07-27},\n\tbooktitle = {Sexual {Reproduction} in {Animals} and {Plants}},\n\tpublisher = {Springer Japan},\n\tauthor = {Sasakura, Yasunori},\n\teditor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},\n\tyear = {2014},\n\tdoi = {10.1007/978-4-431-54589-7_38},\n\tpages = {465--473},\n}\n\n
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\n \n\n \n \n Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T., & Sasakura, Y.\n\n\n \n \n \n \n \n Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona.\n \n \n \n \n\n\n \n\n\n\n Development, 141(2): 481–487. January 2014.\n \n\n\n\n
\n\n\n\n \n \n \"Tissue-specificPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{treen_tissue-specific_2014,\n\ttitle = {Tissue-specific and ubiquitous gene knockouts by {TALEN} electroporation provide new approaches to investigating gene function in \\textit{{Ciona}}},\n\tvolume = {141},\n\tissn = {1477-9129, 0950-1991},\n\turl = {https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by},\n\tdoi = {10.1242/dev.099572},\n\tabstract = {Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill this potential with the advantages of low cost and fast construction times. Here, we report that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis. In Ciona there are well-established methods to introduce exogenous DNA by electroporation, and we show that this method can be used to introduce constructs that can express TALENs ubiquitously or in specific tissues. Our current protocols enable the rapid analysis of hundreds of TALEN-induced mutants. TALEN electroporations result in a high rate of mutations. These mutations can result in gene knockouts that recapitulate previously described functions of Fgf3 and Hox12. We show that TALENs can work efficiently to cause tissue-specific knockouts and demonstrate this by knocking out Hox12 in the epidermis and Fgf3 in neural tissues. We also use tissue-specific knockouts to reveal a new function of Fgf3 during ascidian larval metamorphosis.},\n\tlanguage = {en},\n\tnumber = {2},\n\turldate = {2021-07-27},\n\tjournal = {Development},\n\tauthor = {Treen, Nicholas and Yoshida, Keita and Sakuma, Tetsushi and Sasaki, Haruka and Kawai, Narudo and Yamamoto, Takashi and Sasakura, Yasunori},\n\tmonth = jan,\n\tyear = {2014},\n\tpages = {481--487},\n}\n\n
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\n Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill this potential with the advantages of low cost and fast construction times. Here, we report that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis. In Ciona there are well-established methods to introduce exogenous DNA by electroporation, and we show that this method can be used to introduce constructs that can express TALENs ubiquitously or in specific tissues. Our current protocols enable the rapid analysis of hundreds of TALEN-induced mutants. TALEN electroporations result in a high rate of mutations. These mutations can result in gene knockouts that recapitulate previously described functions of Fgf3 and Hox12. We show that TALENs can work efficiently to cause tissue-specific knockouts and demonstrate this by knocking out Hox12 in the epidermis and Fgf3 in neural tissues. We also use tissue-specific knockouts to reveal a new function of Fgf3 during ascidian larval metamorphosis.\n
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\n \n\n \n \n Yoshida, K., Treen, N., Hozumi, A., Sakuma, T., Yamamoto, T., & Sasakura, Y.\n\n\n \n \n \n \n \n Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases: germ cell mutagenesis in ciona.\n \n \n \n \n\n\n \n\n\n\n genesis, 52(5): 431–439. May 2014.\n \n\n\n\n
\n\n\n\n \n \n \"GermPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
\n 
@article{yoshida_germ_2014,\n\ttitle = {Germ cell mutations of the ascidian \\textit{{Ciona} intestinalis} with {TALE} nucleases: germ cell mutagenesis in \\textit{ciona}},\n\tvolume = {52},\n\tissn = {1526954X},\n\tshorttitle = {Germ cell mutations of the ascidian \\textit{{Ciona} intestinalis} with {TALE} nucleases},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770},\n\tdoi = {10.1002/dvg.22770},\n\tlanguage = {en},\n\tnumber = {5},\n\turldate = {2021-07-27},\n\tjournal = {genesis},\n\tauthor = {Yoshida, Keita and Treen, Nicholas and Hozumi, Akiko and Sakuma, Tetsushi and Yamamoto, Takashi and Sasakura, Yasunori},\n\tmonth = may,\n\tyear = {2014},\n\tpages = {431--439},\n}\n\n
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